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Related post: enhancement of virus replication co-segregates with the viral hemagglutintin and is coded for by the same gene, i.e., gene 4. Finally, using highly specific hyperimmune or monoclonal antibodies, we have Leukeran Cll isolated a series of reassortants Buy Leukeran Online derived from a cross of wild type bovine (UK) or RRV with human rotavirus (serotype 1, 2, 3 or 4). These reassortants that were isolated in primary tissue, possess only one or two human rotavirus genes. One of these genes codes for the glycoprotein that induces neutralizing antibody and thus these reassortants represent prime candidates for use in a live attenuated vaccine. 22-64 PHS 6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00346-02 LID PERIOD COVERED October 1, 1982 through September 30, 1983 TITLE OF PROJECT (80 charactere Chlorambucil Leukeran or Jess. Title muet fit on one line between the borders.) HYBRIDIZATION STUDIES OF ROTAVIRUS PRINCIPAL INVESTIGATOR (List other professional personnel on subsequent pages.) (Name, title, laboratory, and institute affiliation) Jorge Flores, M.D. Expert Consultant LID NIAID COOPERATING UNITS (if any) Hospital de los Ninos, J.M. De Los Rios, Caracas, Venezuela (Dr. Perez, L. White) LAB/BRANCH LABORATORY OF INFECTIOUS DISEASES SECTION EPIDEMIOLOGY SECTION INSTITUTE AND LOCATION NIAID, NIH, BETHESDA, MARYLAND 20205 TOTAL MANYEARS: 0.5 PROFESSIONAL: 0.2 0.3 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects SH (b) Human tissues □({al) Minors □ (a2) Leukeran Cost Interviews □ (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the ipace provided.) We have Leukeran Tablets exploited the ability of rotaviruses to transcribe " in vitro " their genomic RNAs into single stranded (ss) RNAs to prepare radioactive probes that can be used in hybridization assays for detection of rotaviruses and for the analysis of the genetic relatedness among isolates of human and animal origin. 22-65 PHS6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00338-02 LID PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 choractere or less. Title must fit on one line between the borders.) CLONING OF ROTAVIRUS GENES PRINCIPAL INVESTIGATOR (List other professional personnel on subsequent pages.) (Name, title, laboratory, and institute affiliation) Jorge Flores, M.D. Expert Consultant LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH LABORATORY OF INFECTIOUS DISEASES SECTION EPIDEMIOLOGY SECTION INSTITUTE AND LOCATION NIAID, NIH, Buy Leukeran BETHESDA, MD. 20205 TOTAL MANYEARS: 2.2 PROFESSIONAL; 1.2 OTHER; 1.0 CHECK APPROPRIATE BOX(ES) LD (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues CxI (c) Neither SUMMARY OF Leukeran Chlorambucil WORK (Use standard unreduced type. Do not exceed the tpace provided.) We have been able to make DNA copies from genes of three different rotavirus strains (WA, NCDV and Rh2) by reverse transcribing their genomic RNAs or single stranded RNAs synthesized in vitro from rotavirus particles. Tailing of those DNAs and insertion into PBR~T22 plasmid has allowed their cloning into E^. col i . The genes from which most of these clones derive have been identified by means of a dot hybridization assay. Clones containing copies of each rotavirus gene (except for genes 10 and 11) have been identified. The sizes of the rotavirus cDNA inserts into the plasmids have been determined for more than half of all the clones obtained (more than 2500). Rotavirus cDNAs that may represent full size copies of genes 5-9 have been identified and their
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